LAMP Isothermal Amplification PCR Amplification Lucigen.The Omni. Activate Windows 8 Final Version Perfected here. Amp polymerase is the only enzyme capable of Loop mediated Isothermal Amplification LAMP from both RNA and DNA targets.The enzyme has a temperature optimum of 7.C, allowing increased specificity and flexibility compared to Bst Exo Minus or two step processes.The native reverse transcriptase activity of this enzyme means you do not need to add a separate reverse transcriptase for RNA targets.Flexibility The enzyme comes with 1.X Omni.Amp Buffer, Magnesium Sulfate, and Betaine.The buffer is designed to allow for a wide range of additives and easy optimization following the instructions provided in the user manual.Reliability Achieve faster amplification at higher temperatures without sacrificing sensitivity.Absence of Endonuclease or Nicking Activity Incubation of OmniAmp DNA Polymerase with 1 g of supercoiled pBR322 DNA for 16 hours at 70C results in no.Best Software To Design Banners In Photoshop on this page.HighFidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification.Manufactured and qualitycontrolled.The enzyme has high target specificity and low background.Every batch is quality tested to ensure no unwanted nuclease activity will interfere with your sensitive applications.Primer design A robust LAMP reaction depends on proper design of the amplification primers.Please follow this PDF guide to properly use the Primer.Explorer software.DNA polymerases with different features are used for problems arising during DNA synthesis.As an example of asituation when such a problem occurs, it could be.Image/kln%20ktq%20pic.jpg' alt='Exonuclease Activity Of Taq Dna Polymerase Neb' title='Exonuclease Activity Of Taq Dna Polymerase Neb' />The use of six primers is optimal reactions are significantly slower and detection is less sensitive if only four primers are used.Licensing information Lucigen is a fully licensed provider of LAMP reagents for research use.Patents WO 0.WO 0. 13. 47. 90, and WO 0.LAMP method are owned by the Eiken Chemical Co.Ltd.Omni. Amp and Bst Polymerase, Exonuclease minus are sold by Lucigen under license for use in LAMP for research use only.The products may not be used for LAMP based human or diagnostic purposes without obtaining a license from Eiken.US Patent 8.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |